P-34: Crocin Ameliorate The In Vitro Fertilization (IVF) in Mice Treated with Cyclophosphamide

Background Oxidative stress induced by cyclophosphamide (CP), as a chemotherapeutic agent, causes vulnerability of sperm and declining the fertility capacity. Then in this study the effect of crocin (saffron extract) on amelioration of these side effects was investigated. MaterialsAndMethods In this study 24 mice were divided in three groups (n=8). Control group received normal saline (0.2 ml/day, IP), CP group received cyclophosphamide (15 mg/kg/week, IP), and CP+Cr group received crocin (200 mg/ kg/day, IP) along with CP for 35 days.Then, the animals were euthanized for obtaining sperm from the tail of epididymis. After stimulation of ovulation in 72 female adult mice, oocytes were collected and transferred in HTF medium that contained BSA. Then, in vitro fertilization (IVF) was done with capacitated sperms. The rate of fertilization and primitive embryonic growth were evaluated for 120 hours. Results The results showed increase in fertilization, two cell embryos and blastocysts in CP+Cr Group in comparisonto those of CP group (P